Regulatory

Part:BBa_K137127:Design

Designed by: Robert Ovadia   Group: iGEM08_Caltech   (2008-10-16)


A81 Lac Promoter - Tight expression levels


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 115
    Illegal XbaI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 116
    Illegal PstI site found at 130
    Illegal NotI site found at 123
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 116
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
    Illegal SpeI site found at 116
    Illegal PstI site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ordered primers to PCR them out of the original plasmid, but they failed. Decided to order long primers and "dimerized" the promoter with restriction sites at the ends.


Source

Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145.

References